Files selection / Parameters settings
This section and the the following ones address features belonging to the Panel 1 (as defined in the ‘Intro’ section).
Files selection
The images are loaded using the Select Files
buttons associated with each of the Fixed Image and Moving Image boxes.
If the images are oriented differently, it is possible to rotate them by 90°, 180°, or 270° using the Options
tab.

Options
tab.
The Translation
, Rotation
, Scaling
, and Shearing
options allow enabling or disabling the types of transformations considered within the transformation matrix.
The alignment is performed in pairs. By consequence the number of selected images must meet the following criteria:
N-to-N Alignment: The number of Fixed images must match the number of Moving images. In this case, the images will be processed pairwise in the order in which they appear in their respective file lists.
1-to-N Alignment: There must be exactly one Fixed image and any number of Moving images. In this case, all Moving images will be aligned to the single Fixed image provided.

Illustration of different files selection use cases.
Defining ROIs
In addition to defining ROIs (regions of interest) using the mouse ni the main Figure window (Panel 4), they can also be specified textually in the related fields, following the convention: [xmin, xmax, ymin, ymax], based on the coordinate system of the image displayed in Panel 4.
